This study is aimed at assessing the level of knowledge of symptoms, signs and risk factors of Prostate cancer among male senior staff of University of Uyo and Teaching Hospital. Data from this descriptive cross sectional study were collected through a self-administered questionnaire to consenting male senior staff of University of Uyo and University of Uyo Teaching Hospital over a period of one (1) month in 2021. There was a total of 182 respondents. The mean age was 50.5 years with the majority of respondents being within 51- 60 years age group (50.0%). Majority of the respondents were University Lecturers (61.5%). A total of 70 respondents were knowledgeable of the warning symptoms and signs of Prostate cancer (38.5%). Staff of Teaching hospital were more knowledgeable about the warning symptoms and signs of Prostate cancer than staff of the University of Uyo (21.5% vs 17.0%). Nocturia was the leading worrisome symptom of change in urinary pattern acknowledged by the respondents (42.9%). Acute urinary retention was the leading recognized symptomatic complication (50.0%). Over thirty percent (31.3%) of respondents were knowledgeable of the risk factors for Prostate cancer with a larger proportion of respondents correctly acknowledging a red meat enriched diet as the main contributory factor (24.6%). University staff were more knowledgeable about risk factors relative to
those in the Teaching hospital (52.6% vs 47.4%). In conclusion knowledge about Prostate cancer warning signs and risk factors was poor.